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Chromatography Resins

Protein A Media

Cellufine SPA-HC

Cellufine SPA-HC is categorized as Protein A media for antibody purification. Protein A media is usually selected as the first of two steps in antibody purification chromatography and proves to be crucial for the overall purification process. Cellufine SPA-HC is a high-performance media which allows for purification of a large volume of culture supernatant in short period of time. The resin provides:

  • High dynamic binding capacity at a high flow rate.
  • Low back pressure at a high flow rate.
  • Reusability.
  • Overview

    Cellufine SPA-HC is the resin immobilized alkali resistance protein A ligand. The resin features low back pressure attributed to its crosslinking technology and has high porosity in order to achieve high mass transfer of antibodies. The resin is created using unique bead manufacturing technology developed through 40th years of business in chromatography, guarantying high dynamic binding capacity at high flow rates.

  • Characteristics
    Property Characteristics
    Ligand Alkali-stable rProtein A affinity ligand produced in E.coli
    Matrix Highly cross-linked cellulose beads
    Particle size Average 70 µm diameter
    Ligand coupling method Coupling via formyl groups on resin
    Flow velocity ≧650 cm/h (0.3 MPa) with 10 cmID x 20 cmL Column in water at 24 ℃
    Dynamic binding capacity (DBC) ≧ 70 mg /ml (DBC C10 with Polyclonal IgG)
    Recommend CIP 0.1 M NaOH
    Temperature stability 4 - 50 ℃ No significant change in performance after 1-week storage.
    Storage 2 - 8 ℃ in 20 % (v/v) ethanol
    Chemical stability No significant change in performance after 1-week storage in 30 % (v/v) isopropanol, 20 – 70 % (v/v) ethanol, 8 M Urea, 6 M Guanidine-HCl or 0.1 M Acetic acid.
    pH working range 3-12
  • Features
    • Coupled with an alkali resistance rProtein A ligand
    • Excellent dynamic binding capacity
    • Low back pressure at high flow rate
    • Robust performance independent of monoclonal antibody
    • Reduced bead diameter
    • Excellent reusability
  • Related Documents

Affinity Chromatography Media

Cellufine Sulfate

Advances in vaccines and clinical diagnostics have created an increasing demand for large volumes of highly purified and concentrated virus and viral or microbial antigens. Cellufine Sulfate affinity media is a simple, rapid and effective means for concentration, purification and depyrogenation of these important products. Cellufine Sulfate eliminates cumbersome, time-consuming and potentially unsafe classical ultra-centrifugation and density gradient methods. It can also provide a significant improvement in concentration and purity.

Cellufine MAX DexS-HbP, Cellufine MAX DexS-VirS

We offer two different Cellufine MAX DexS resins, surface modified with different lengths of Dextran Sulfate polymer; a) DexS-HbP developed for purification of heparin binding proteins. and b) DexS-VirS, for purifying virus and virus like particles especially having affinity for heparin. Both resins have been developed to improve the protein or virus capture efficiency of existing resins by employing a pseudo affinity mimetic polymer surface based on Dextran Sulfate. The cross-linked cellulose base bead has been optimized for high flow applications and is fully compatible up to 0.5 M NaOH for CIP.

Cellufine ET clean L & Cellufine ET clean S

The Cellufine™ ET clean media are poly(ε-lysine) immobilized Cellufine™ (cellulose spherical beads) and are categorized as Affinity Chromatography Media for Endotoxin Removal. These beads efficiently bind and remove endotoxins from your sample solution. The poly(ε-lysine) is a microbial poly(amino acid) that consist of 25-35 lysine residues produced by Streptomyces albulus. The poly(ε-lysine) act as the ligand and the cellulose beads act as matrix.

Cellufine™ PB

Cellufine PB is an affinity medium designed for concentration, purification of glycoprotein, glycated protein, saccharide. This media is made of spherical, rigid cellulose beads functionalized with phenyl borate. The phenyl borate groups give unique chromatographic selectivity for cis-diol groups of the target molecule.

Cellufine Phosphate

Cellufine Phosphate is an affinity media designed for concentration, purification of proteins and, enzymes such as nucleic acid related proteins. The base of the media is spherical and rigid cellulose functionalized with Phosphate esters. Cellufine™ Phosphate belongs to the new Cellufine™ series and has spherical porous beads. Unlike the conventional resin made from fiber, Cellufine Phosphate resin is able to withstand high pressure and is consequently used in large-sized columns with high flow rate.

Cellufine Formyl

The growth of process-scale affinity chromatography has created the need for a new generation of support matrix materials and coupling chemistries suited for the industrial environment. Classical agarose-based supports perform poorly at the large-scale for several reasons. They provide poor flow properties in large columns. The widely used cyanogen bromide coupling chemistry has well-documented problems with bond stability and non-specific adsorption. Additionally, even with more modern chemistries, agarose can shed polysaccharide chains, giving rise to significant ligand leakage under mild operating conditions. Cellufine activated supports provide state-of-the-art laboratory performance at the process-scale without difficulty. The products are based on rigid spherical cellulose beads specially optimized for affinity chromatography to provide very large pore size and high ligand capacity together with high flow rates in large columns. The cellulose backbone offers very low non-specific adsorption without the ligand leakage problems of agarose.

  • Characteristics
    Characteristics
    Substrate Crosslinked cellulose
    MW Exclusion Limit 4,000kD
    Standard Particle Size 125 - 210µm
    Particle Shape Spherical
    Density 0.7g/ml wet
    Shrinkage / Swelling Will not shrink or swell substantially under changes in pH or ionic strength
    Chemical Resistance Can be used with any salts, non-ionic detergents, organic solvents. Resistant to 0.1M HCl and 0.5M NaOH. (Note: coupled ligand may not be stable under these conditions)
    Mechanical Resistance Will withstand peristaltic pumping and extended mixing
    Autoclavable 121 °C for 30 minutes at pH 7
    Saturation Capacity Up to 40mg protein/ml depending upon protein and conditions
    Operating Pressure < 1 bar (15 psi)
    Supplied Formyl 0.01 % 2, 2-thio-bis (pyridine-1-oxide)
  • Features
    • High flow rates in laboratory and process columns for high throughput
    • Low ligand leakage
    • Excellent mechanical, chemical and environmental resistance
    • High ligand loading capacity
    • Compatible with high molecular weight ligands and target proteins due
    • Unreacted formyl groups easily converted during reduction to neutral hydroxyls
    • Built in hydrophilic spacer arms for maximum ligand accessibility and low non-specific adsorption
    • No media damage or fines generation with extended mixing
    • Thermal stability of media allows high temperature reactions
    • Long shelf-life of unreacted media
  • Related Documents

Hydrophobic Interaction Chromatography Media

Cellufine™ MAX Phenyl & Cellufine™ MAX Phenyl LS

Cellufine MAX Phenyl is useful for the chromatography of hydrophobic proteins. Many proteins have hydrophobic amino acid residues which will interact with the phenyl functional groups. Factors that influence this hydrophobic interaction include salt concentration, temperature, pH, organic solvents and surfactants. Protein adsorption usually occurs at high ionic strength, while elution occurs at lower salt concentrations. This is the opposite of ion exchange chromatography and offers complementary separation benefits.

Cellufine™ MAX Butyl

Cellufine MAX Butyl is useful for the chromatography of hydrophobic proteins. Many proteins have hydrophobic amino acid residues which will interact with the butyl functional groups. Factors that influence this hydrophobic interaction include salt concentration, temperature, pH, organic solvents and surfactants. Protein adsorption usually occurs at high ionic strength, while elution occurs at lower salt concentrations. This is the opposite of ion exchange chromatography and offers complementary separation benefits.

Ion Exchange Chromatography Media (IEX)

Cellufine A-200, A-500 and A-800

Cellufine A-200, A-500 and A-800 media are designed for the anion exchange chromatography of proteins, peptides and other biomolecules. The media are comprised of beaded spherical cellulose, functionalized with DEAE (diethylaminoethyl). The pore size and structure of each packing determines its respective applications. Cellufine A-200 medium is ideal for the chromatography of low molecular weight peptides or proteins (< 30kD), A-500 for proteins up to 500 kD and A-800 for use with larger biomolecules up to 1000 kD. The superior rigidity of Cellufine media allows for high flow rates, and thus, rapid processing times.

Cellufine C-500

Cellufine C-500 media manufactured by JNC Corporation are designed for the cation exchange chromatography of basic proteins and other biomolecules. The resins are comprised of beaded spherical cellulose, functionalized with carboxymethyl (CM). Cellufine C-500 medium is ideal for the chromatography of proteins molecular weight up to 500 kD. The superior rigidity of Cellufine gels allows for high flow rates, and thus rapid processing times, even in large diameter process scale columns.

Cellufine Q-500

Cellufine Q-500 media is designed for the anion exchange chromatography of acidic proteins, peptides and other biomolecules. The resins are comprised of beaded spherical cellulose, functionalized with a quaternary amine (trimethy-laminoethyl). The pore size and structure of each media determines its respective applications. Cellufine Q-500 medium is ideal for molecules up to 500 kD. The improved rigidity of Cellufine allows for high flow rates, and thus, rapid processing times, even in large diameter process scale columns.

Cellufine S-500

Cellufine S-500 media manufactured by JNC are designed for the cation exchange chromatography of basic proteins and other biomolecules. The resins are comprised of beaded spherical cellulose, functionalized with sulfobutyl as the ligand. Cellufine S-500 medium is ideal for the chromatography of proteins with molecular weight up to 500 kD. The superior rigidity of Cellufine gels allows for high flow rates, and thus rapid processing times, even in large diameter process scale columns.

Ion Exchange Chromatography Media MAX (IEX MAX)

Cellufine MAX S-r & Cellufine MAX S-h

Cellufine MAX is the new name of 2nd generation Cellufine chromatography media. Cellufine MAX S strong ion exchangers are highly cross-linked, surface modified media with high dynamic binding capacities and stable at high flow velocities. These high performances of the optimized media offer significant opportunity for increasing downstream purification throughput. The characteristics of Cellufine MAX “-r” is high recovery, high resolution and robustness. The characteristic of Cellufine MAX “-h” is the highest adsorption capacity in present products.

Cellufine MAX Q-r & Cellufine MAX Q-h

Cellufine MAX is the new 2nd generation Cellufine chromatography media. Cellufine MAX Q strong ion exchangers are highly cross-linked, surface modified media with high dynamic binding capacities and stability at high flow velocities. These high-performance optimized media offer significant opportunity for increasing downstream purification throughput. The characteristics of Cellufine MAX “-r” sires are high recovery, high resolution and robust. The characteristic of Cellufine MAX “-h” is the highest adsorption capacity in present products.

Cellufine MAX CM

Cellufine MAX is the new 2nd generation Cellufine chromatography media. Cellufine MAX CM weak cation exchanger is highly cross-linked, surface modified media with high dynamic binding capacities and stable at high flow velocities. These high performances of the optimized media offer significant opportunity for increasing downstream purification throughput.

Cellufine MAX DEAE

Cellufine MAX is the new 2nd generation Cellufine chromatography media. Cellufine MAX DEAE weak anion exchanger is highly cross-linked, surface modified media with high dynamic binding capacities and stable at high flow velocities. These high performances of the optimized media offer significant opportunity for increasing downstream purification throughput.

Cellufine MAX S-r & Cellufine MAX S-h

Cellufine MAX is the new name of 2nd generation Cellufine chromatography media. Cellufine MAX S strong ion exchangers are highly cross-linked, surface modified media with high dynamic binding capacities and stable at high flow velocities. These high performances of the optimized media offer significant opportunity for increasing downstream purification throughput. The characteristics of Cellufine MAX “-r” is high recovery, high resolution and robustness. The characteristic of Cellufine MAX “-h” is the highest adsorption capacity in present products.

Cellufine MAX GS

Cellufine MAX is the new 2nd generation Cellufine chromatography media. Cellufine MAX GS strong cation exchanger is highly cross-linked media with stable at high flow velocities and with optimized ligand density. These high performances offer significant opportunity for increasing downstream purification throughput, especially aggregate removal from therapeutic molecular antibodies.

Mix Mode Chromatography Media

Cellufine MAX AminoButyl

Mixed mode chromatography media are well-known to have unique selectivity differences from traditional IEX or HIC resins. JNC corporation has developed a new mix-mode resin, Cellufine MAX AminoButyl, for virus or virus like particles (VLPs) purification. This resin has both salt tolerant primary amine and hydrophobic butyl group as ligands. Especially ligand density of butyl group is optimized for adsorption/desorption of VLPs having strong hydrophobicity. This mixed mode resin is built on the cross-linked Cellufine cellulose base bead that is very stable, resistant to base CIP and can be operated under high flow modes with minimal back pressure.

Cellufine MAX IB

Mixed mode chromatography resins are well-known to have unique selectivity differences from traditional IEX or HIC resins. JNC Corporation has developed a new mix-mode resin, Cellufine™ MAX IB, for monoclonal antibody (Mab) purification after initial rProtein A capture. This resin has a salt tolerant polyallylamine surface modification that has been partially modified with butyl groups. The resulting ligand structure exhibits a mixed mode functionality with a hybrid primary amine + butyl surface chemistry. In addition to the Mab polishing application this resin can be used in other downstream processes, such as plasma fractionation exploiting the unique selectivity of this mixed-mode ligand. This mixed mode resin is built on the cross-linked Cellufine cellulose base bead that is very stable, resistant to base CIP and can be operated under high flow modes with minimal back pressure.

Gel Filter Action Chrom Media

Cellufine™ GCL-2000 & GCL-2000HF

Gel Filtration, or size exclusion chromatography, has proven to be a simple and effective purification method for proteins and other biomolecules. However, the traditionally slow flow rates and limiting resolving power of most gel filtration media have restricted its usefulness. JNC’s Cellufine GCL media has been designed to overcome these limitations. The mechanical strength of the spherical cellulose matrix allows high flow rates, even in large industrial columns

Cellufine GH-25

Cellufine GH-25 provides a rapid means of salt removal and buffer exchange for protein solutions. The semi-rigid spherical cellulose beads allow high flow rates with little compression of the column bed. The separation mechanism is based on differential solute access into the chromatographic bead. Whereas large molecules (above 3 kD) are excluded from the packing and pass quickly through the column, small molecules (salts) diffuse into the bead and are thus retained longer. GH-25 can be used to remove alcohols, salts, detergents, fluorochromes, sugar, etc., from virtually any protein solution. It is compatible with most solvents and is stable from pH 1 - 14.

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